Guy Guillemette

Guy Guillemette
Professor Emeritus
Status: Emeritus


Nitric Oxide (NO) is an important regulator of numerous essential physiological processes like blood pressure, neurotransmission, insulin release, memory storage and the immune response. In addition, NO is involved in a variety of serious pathophysiological processes including septic shock, atherosclerosis, diabetes, as well as myocardial and cerebral ischemia. The biogenesis of NO is catalyzed by Nitric Oxide Synthase (NOS) enzymes. The long-term goal of our research program is to understand the regulation and mechanism of the NOS enzymes.

Research Interests

  • Protein structure and function
  • nitric oxide synthase
  • calmodulin
  • enzymology
  • biophysics


  • 1988 Ph.D., Biochemistry, University of Toronto, Canada
  • 1981 B.Sc., Biochemistry, University of Toronto, Canada


  • 1993-1997 FRSQ Chercheur-Boursier ($43,000/yr towards salary) Investigation of the function of the pyruvate dehydrogenase enzyme. Declined after June 30, 1995
  • 1986 Scholarship from the Huntington’s Society of Canada
  • 1985 OGS scholarship
  • 1984 Scholarship from the Huntington’s Society of Canada


  • 2012-2015 Guelph-Waterloo Centre for Graduate Work in Chemistry and Biochemistry (GWC)2 Director
  • 2012-2015 Science Faculty Council
  • 2015 Chemistry Safety committee
  • 2009-2012 Graduate Officer
  • 2009-2012 IBMB Director
  • 2009-2012 Graduate advisor committee
  • 2005-2012 (GWC)2 Coordinating Committee
  • 2003-2012 Undergraduate Relations Committee

Professional Associations

  • 2009-present, Editorial Board member, Biochemical Research Letters
  • 2008-present, Journal of Biomedicine and Biotechnology

Affiliations and Volunteer Work

  • Member, Institute of Biochemistry & Molecular Biology


  • CHEM 233 - Fundamentals of Biochemistry
    • Taught in 2020
  • MNS 331 - Biomaterials
    • Taught in 2019, 2020

* Only courses taught in the past 5 years are displayed.

Selected/Recent Publications

  • Pimlott, Douglas, Guy Guillemette, and Thorsten Dieckmann. "Structural Investigation of Melatonin Metabolites as Calmodulin Antagonists and potential Nitric Oxide Synthase Inhibitors." The FASEB Journal 33.1_supplement (2019): 784-12.
  • Piazza M., Dieckmann T., Guillemette J.G. (2018). Investigation of the structure and dynamic of calmodulin-nitric oxide synthase complexes using NMR spectroscopy. Front. Biosci. 23, 1902-1922.
  • Piazza M, Taiakina V., Dieckmann T, Guillemette JG (2017). Structural consequences of Calmodulin EF muations. Biochemistry 56, 944-956.
  • Piazza, M., Dieckmann, T., Guillemette, J.G. (2016). StructuralStudies of a Complex between endothelial Nitric Oxide Synthase and Calmodulin at Physiological Calcium Concentration. Biochemistry 55, 5962- 5971.
  • Blondeel, E.J.M., Ho, R., Schulze, S., Sokolenko,, S., Guillemette, S., Slivac, I., Durocher, Y., Guillemette, J.G., McConkey, B., Chang, D., Aucoin, M.G. (2016). An Omics Approach to Rational Feed: Enhancing Growth in CHO Cultures with NMR Metabolomics and 2D-DIGE Proteomics Journal of Biotechnology. Journal of Biotechnology 234, 127-138.
  • Piazza, M., Guillemette. J.G., Dieckmann T. (2016). Chemical shift assignments of calmodulin constructs with EF hand mutations. Biomol. NMR Assign. 10, 193-198.
  • Piazza, M., Guillemette, J.G., Dieckmann, T. (2015) Chemical shift perturbations induced by residue specific mutations of CaM interacting with NOS peptides. Biomolecular NMR Assigments 9(2) 299-302.


  • Patent Application – “Spatio-Temporal Activation, Site Selection, and Monitoring of Treatment Agent and Activity Thereof Using EMR”, Jervis E.J., Guillemette J.G., Perdicakis B., Montgomery H.J., Lajoie G.A. Fishlock D., US and European Patent Applications Filed Oct 12, 2005.

Graduate studies