MASc Oral Exam| Enzyme-mediated controlled release of DNA polyplexes from gelatin methacrylate hydrogel for nonviral gene delivery by Joshua Kunihiro

In this MASc oral exam, Joshua Kunihiro will be discussing their research into  Enzyme-mediated controlled release of DNA polyplexes from gelatin methacrylate hydrogel for nonviral gene delivery

Abstract:

The objective of this project was to demonstrate the potential of GelMA hydrogel as an enzyme-responsive controlled release scaffold for chitosan-graft-polyethyleneimine (CS-PEI) polyplexes as a non-viral gene delivery platform to treat corneal diseases or skin wounds. The aims of this work were to characterize the tunable release kinetics of the polyplexes and assess the bioactivity of polyplexes released from the GelMA hydrogel system by in vitro transfection efficiency.

Supervisor: Professor; Yim