ABSTRACT:
The
Regenerative
Medicine
and
Skeleton
laboratory
(INSERM
U1229,
Nantes,
France)
has
developed
an
injectable
and
self-hardening
silated-HydroxyPropylMethyl
Cellulose
(Si-HPMC)
hydrogel.
In
this
talk,
we
will
go
through
our
recent
approaches
using
Si-HPMC
hydrogels
as
a
drug
and/or
cell
carrier
in
the
context
of
degenerative
diseases.
Intervertebral
disc
(IVD)
disease
is
a
major
cause
of
low
back
pain
and
a
low-invasive
approach
for
intradiscal
delivery
of
therapeutics
is
of
particular
interest.
We
demonstrated
the
role
of
Growth
Differenciation
Factor-5
as
a
nucleopulpogenic
growth
factor
to
induce
differentiation
of
human
stromal
cells
into
disc
cells
and
we
are
now
evaluating
its
in
vitro
sustained
release
as
well
as
its
ability
to
promote
in
situ
regenerative
process.
Osteoarthritis,
a
joint
disease
in
which
cartilage
degeneration
goes
along
with
synovium
inflammation,
could
benefit
from
the
ability
of
Mesenchymal
Stromal
Cells
(MSCs)
to
secrete
anti-inflammatory
and
immuno-modulatory
factors.
Considering
cell
leakage/death
upon
intra-articular
injection,
we
propose
to
embed
MSCs
in
a
cytoprotective
hydrogel
to
extend
their
local
retention
and
long-term
clinical
efficacy.
We
have
compared
several
methods
(dripping,
emulsification,
soft
lithography)
to
prepare
hydrogel-based
particles
with
a
controlled
size
and
demonstrated
cell
viability
after
encapsulation,
as
well
the
secretion
of
secrete
therapeutic
factors
(PGE2,
IDO,
HGF).
Bio-Sketch:
Catherine
Le
Visage
is
currently
a
Research
Director
and
the
Deputy
Director
of
INSERM
UMR
1229/Regenerative
Medicine
and
Skeleton
(RMeS)
laboratory
(University
of
Nantes,
France).
She
was
trained
as
a
Pharmacist
and
she
received
her
PhD
in
Pharmaceutical
Technologies
from
University
Paris
11.
She
then
performed
a
post-doctoral
training
at
the
Johns
Hopkins
School
of
Medicine
(USA)
in
Prof
Kam
Leong’s
laboratory
with
a
focus
on
thermo-sensitive
hydrogels
for
stem
cell
delivery.
In
2007,
she
joined
the
French
National
Institute
of
Health
and
Medical
Research
to
investigate
chemically
cross-linked
polysaccharides
hydrogels
as
platforms
for
regenerative
medicine.
Currently,
her
interest
lies
in
developing
hydrogels
for
long-term
delivery
of
biochemical
cues
in
the
context
of
soft
tissues
such
as
intervertebral
disc
disease
and
osteoarthritis.
She
has
coordinated
a
France-Singapore
ANR
project
on
functionalized
hydrogels
and
has
been
a
co-PI
in
multiple
national
grants
on
cell
therapy
and
regenerative
medicine.
She
has
authored
49
publications
in
ISI-indexed
journals
(h-index
21)
and
11
patents.
Monday, March 27, 2017 11:30 am
-
11:30 am
EDT (GMT -04:00)