ABSTRACT: In this work we present results on the use of Gamma-Polyglutamic Acid (PGA) biopolymer to produce nanoparticles and encapsulate the anti-cancer drug Doxorubicin (DOX). Microbially produced PGA is used alone and in combination with Chitosan (CS) to form DOX-loaded nanoparticles at different conditions. The main mechanism of nanoparticle formation is the ionic complexation between PGA, DOX and CS. The size distribution, surface charge and diffusivity characteristics of PGA-DOX-CS nanoparticles were studied under the following variable conditions: order and time of mixing, mass ratios of PGA:DOX:CS, structure and molecular weight of components, and pH of solution. Rates of biodegradation of nanoparticles and rate of DOX release were studied as a function of time. Preliminary in-vitro cytotoxicity results are also reported using different size nanoparticles to kill human cancer cells HN-5a derived from Human gingival squamous carcinoma.
B.A.Sc. Chemical Engineering, University of Waterloo, Canada
M.S. Chemical Engineering (Food Eng.), University of California, Berkeley, U.S.A.
Ph.D. Biochemical Engineering, University of California, Berkeley, U.S.A.
TEACHING: Biochemical Engineering, Bioprocessing, Bioseparation, Selected Topics in Biochemical Engineering, Bio-Environmental Engineering, Sustainability and Environment
RESEARCH AREAS: Biochemical and Biomedical Engineering, Bio-Environmental Engineering, Biofuels (ethanol, butanol and hydrogen gas), Biopolymers, Bioprocess Design, Controlled Release of Pharmaceuticals from Nanoparticles, Fermentation, Fluidized Bioreactors, Supercritical CO2 Extraction of Biopharmaceuticals and Nutraceuticals.