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Using CRISPR/Cas9 to screen the Autographa Californica multiple nucleopolyhedrovirus genome for essential and non-essential genes using an HIV-GAG Virus Like Particle under the p6.9 promoter

Enabling technology platforms for tissue engineering and regenerative medicine research

CHAN PUI BARBARA

SBS, iTERM, BME, CUHK

The research focus of the Tissue Engineering Lab at CUHK centers around bioengineering of cell- and biomaterial-based complex living tissue substitutes for tissue engineering and regenerative medicine applications. During the prolonged journey of developing engineered tissues for regenerative medicine purposes, we have faced many technical challenges associated with the major components of engineered tissues including stem cells, biomaterials and cell niche signals. A handful of technology platforms were developed to enable and facilitate research in tissue engineering and regenerative medicine research, including but are not limited to (1) a multiphoton microfabrication and micropatterning (MMM) technology to define the cell niche interactions; (2) a multi-level mechano-regulation (MMR) platform to facilitate mechano-characterization and manipulation of cells and tissues; and (3) a biomimetic biomacromolecular microencapsulation (BBM) platform to facilitate physiologically relevant scaffolding. In this seminar, the rationales, the technological capability and the relevant applications will be discussed.