Grad Seminar - Multi-Wavelength in vivo Photon Absorption Remote Sensing: Towards Non-Contact Label-Free Functional Vascular Imaging
Abstract
Functional vascular metrics, such as oxygen saturation (SO2), are highly valuable for diagnosis and treatment in oncology and ophthalmology. This work demonstrates the first in vivo multi-wavelength Photon Absorption Remotes Sensing (PARS) system. Implementing two excitation wavelengths leverages the varying absorption spectra for oxy- and deoxy-hemoglobin, laying the groundwork for SO2 measurement. New system developments for SO2 measurement includes independent excitation paths, pulse-specific power compensation, and polarization alignment to further work towards a PARS oximeter that achieves ISO accuracy standards. This work demonstrates repeatable and consistent in vivo images of chicken embryo vasculature. Additionally, the development of an in vitro blood oxygenation and deoxygenation protocol, and the design of a flow phantom is explored. Finally, functional change in blood oxygenation is demonstrated through an in vivo hypoxia study.
Presenter
Sarah Werezak, MASc candidate in Systems Design Engineering
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