Overview
The Waterloo Genomics Surveillance Centre (WGSC) provides genomic sequencing and analytical services in support of surveillance and research activities. These services include library preparation, sequencing, and bioinformatics support. The WGSC is a centralized Core Research Facility under the Office of Research located in the Biology Department.
The WGSC provides access to Illumina (MiSeq & NextSeq) and Oxford Nanopore Technologies (MinION) sequencing platforms as well as provides technical support for library preparation and QA/QC processes. The WGSC has specialized equipment to aid in the preparation (e.g. thermocyclers, automated extraction equipment), QA/QC (gel electrophoresis, fluorometer), as well as necessary general laboratory equipment necessary to conduct molecular biology research. For more information please visit the confluence page.
Current Services Provided:
Illumina NextGen Sequencing (MiSeq and NextSeq 2000)
Illumina Library Preparation for:
- 16S/18S/ITS Amplicon Sequencing
- Whole Genome/Metagenomic Sequencing
- mRNA Sequencing
- Total RNA with Ribodepletion Sequencing
- Small RNA Sequencing
Oxford Nanopore Technology (ONT) Sequencing (MinION and Flongle)
ONT Library Preparation using:
- Rapid Barcoding Kits
- Native Barcoding Kits
- cDNA-PCR Barcoding Kits
ONT Applications:
- Whole Genome/Metagenomic Sequencing
- Plasmid Sequencing
- Amplicon Sequencing
- Transcriptomics
QA/QC
Agilent 4150 Tapestation analysis:
- DNA
- High Sensitivity DNA
- Genomic DNA
- RNA
- High Sensitivity RNA
Fluorometric Quantification (e.g. Qubit Analysis)
- dsDNA (High Sensitivity, Broad Range)
- RNA (High Sensitivity, Broad Range and Extended Range)
For further information and pricing please contact genomics@uwaterloo.ca or visit the confluence page (sign-in maybe required).