female student working in lab

Molecular Biology Core Facility

Overview

This facility, supervised by Dr. Andrew C. Doxey, provides state-of-the-art imaging and analysis equipment for students and faculty to use in their research. These items are usually not available in individual laboratories, so their presence in a shared facility enhances the graduate experience for our students. In addition, the molecular core facility has a set of basic molecular biology equipment that is universally available so that students who are not in molecular biology oriented labs who might need such equipment for a small part of their project have access to what they need.

Equipment available

Our service includes the following

  1. MBCF core facility
  2. NEB Biobar
  3. MiSeq genome sequencing
  4. FACS (in service as of March 2015)
  5. Service hours: Monday to Friday, 8am  to 4pm

MBCF service charges

  1. MBCF basic service charge: $30 per month (including Milli-Q water, Nano drop machine, Gel documentation system, centrifuge, freezer alarm system, cotton for serological pipets, etc.)
  2. $20 per 24hours (minimum) for freeze dryer.
  3. Freezer rental: $50 per compartment per month (ranging from 10 x 30 to 17 x 30 inches), $25 per half compartment per month.
  4. Flow cytometry: Please refer to the FACS webpage.
  5. Freezer alarm system: Biology department covers the cost.
  6. Cotton for serological pipets: (commercial disposable pipet cost @ $0.26 per pipet) (cotton cost $296.23 for 2lb): Biology department covers the cost.

MiSeq service and maintenance charges

Pricing Chart
Item Description Cat # Cost
MiSeq Reagent Kit v2 (50 cycles) MS-102-2001 $275
MiSeq Reagent Kit v2 (300 cycles) MS-102-2002 $320
MiSeq Reagent Kit v2 (500 cycles) MS-102-2003 $365
MiSeq Reagent Micro Kit v2 (300 cycles) MS-103-1002 $235
MiSeq Reagent Nano Kit v2 (300 cycles) MS-103-1001 $230
MiSeq Reagent Nano Kit v2 (500 cycles) MS-103-1003 $320
MiSeq Reagent Kit v3 (150 cycles) MS-102-3001 $300
MiSeq Reagent Kit v3 (600 cycles) MS-102-3003 $400

PhiX control cost $12.75 per uL.

Contact information

For MBCF policy please contact

Dr. Andrew Doxey

Molecular Core – Director
University of Waterloo
Biology 1, Room 166A
Waterloo, Ontario N2L 3G1
Telephone: (519) 888-4567 ext. 33889
Fax: (519) 746-0614
Email: acdoxey@uwaterloo.ca

For technical and service please contact

Akash Jairaj

Molecular Core – FACS Specialist
University of Waterloo
Biology 2, Room 357A
Waterloo, Ontario N2L 3G1
Telephone: (519) 888-4567 ext. 33526
Fax: (519) 746-0614
Email: ajairaj@uwaterloo.ca