Overview
This facility, supervised by Dr. Andrew C. Doxey, provides state-of-the-art imaging and analysis equipment for students and faculty to use in their research. These items are usually not available in individual laboratories, so their presence in a shared facility enhances the graduate experience for our students. In addition, the molecular core facility has a set of basic molecular biology equipment that is universally available so that students who are not in molecular biology oriented labs who might need such equipment for a small part of their project have access to what they need.
Equipment available
Our service includes the followings:
- MBCF core facility
- NEB Biobar
- MiSeq genome sequencing
- FACS (in service as of March 2015)
- Service hours: Monday to Friday, 8am to 4pm
MBCF service charges:
- MBCF basic service charge: $30 per month (including Milli-Q water, Nano drop machine, Gel documentation system, centrifuge, freezer alarm system, cotton for serological pipets, etc.)
- $20 per 24hours (minimum) for freeze dryer.
- Freezer rental: $50 per compartment per month (ranging from 10 x 30 to 17 x 30 inches), $25 per half compartment per month.
- Flow cytometry: Please refer to the FACS webpage.
- Freezer alarm system: Biology department covers the cost.
- Cotton for serological pipets: (commercial disposable pipet cost @ $0.26 per pipet) (cotton cost $296.23 for 2lb): Biology department covers the cost.
MiSeq service and maintenance charges:
| Item Description | Cat # | Cost |
|---|---|---|
| MiSeq Reagent Kit v2 (50 cycles) | MS-102-2001 | $275 |
| MiSeq Reagent Kit v2 (300 cycles) | MS-102-2002 | $320 |
| MiSeq Reagent Kit v2 (500 cycles) | MS-102-2003 | $365 |
| MiSeq Reagent Micro Kit v2 (300 cycles) | MS-103-1002 | $235 |
| MiSeq Reagent Nano Kit v2 (300 cycles) | MS-103-1001 | $230 |
| MiSeq Reagent Nano Kit v2 (500 cycles) | MS-103-1003 | $320 |
| MiSeq Reagent Kit v3 (150 cycles) | MS-102-3001 | $300 |
| MiSeq Reagent Kit v3 (600 cycles) | MS-102-3003 | $400 |
PhiX control cost $12.75 per uL.
The Molecular Biology Core Facility (MBCF) Equipment Booking
Contact information
For MBCF policy please contact:
Dr. Andrew Doxey
Molecular Core – Director
University of Waterloo
Biology 1, Room 166A
Waterloo, Ontario N2L 3G1
Telephone: (519) 888-4567 ext. 33889
Fax: (519) 746-0614
Email: acdoxey@uwaterloo.ca
For technical and service please contact:
Akash Jairaj
Molecular Core – FACS Specialist
University of Waterloo
Biology 2, Room 357A
Waterloo, Ontario N2L 3G1
Telephone: (519) 888-4567 ext. 33526
Fax: (519) 746-0614
Email: ajairaj@uwaterloo.ca