Molecular Biology core facility

Overview

This facility, supervised by Dr. Andrew C. Doxey, managed by Dr. Terence Tang, provides state-of-the-art imaging and analysis equipment for students and faculty to use in their research. These items are usually not available in individual laboratories, so their presence in a shared facility enhances the graduate experience for our students. In addition, the molecular core facility has a set of basic molecular biology equipment that is universally available so that students who are not in molecular biology oriented labs who might need such equipment for a small part of their project have access to what they need.

Equipment available

Our service includes the followings:

  1. MBCF core facility
  2. NEB Biobar
  3. MiSeq genome sequencing
  4. FACS (in service as of March 2015)
  5. Service hours: Monday to Friday, 8am  to 4pm

MBCF service charges:

  1. MBCF basic service charge: $30 per month (including Milli-Q water, Nano drop machine, Gel documentation system, centrifuge, freezer alarm system, cotton for serological pipets, etc.)
  2. $10 per use for scintillation counter
  3. $20 per 24hours (minimum) for freeze dryer.
  4. Freezer rental: $50 per compartment per month (ranging from 10 x 30 to 17 x 30 inches), $25 per half compartment per month.
  5. Flow cytometry: Please refer to the FACS webpage.
  6. Freezer alarm system: Biology department covers the cost.
  7. Cotton for serological pipets: (commercial disposable pipet cost @ $0.26 per pipet) (cotton cost $296.23 for 2lb): Biology department covers the cost.

MiSeq service and maintenance charges:

Item Description Cat # Cost
MiSeq Reagent Kit v2 (50 cycles) MS-102-2001 $275
MiSeq Reagent Kit v2 (300 cycles) MS-102-2002 $320
MiSeq Reagent Kit v2 (500 cycles) MS-102-2003 $365
MiSeq Reagent Micro Kit v2 (300 cycles) MS-103-1002 $235
MiSeq Reagent Nano Kit v2 (300 cycles) MS-103-1001 $230
MiSeq Reagent Nano Kit v2 (500 cycles) MS-103-1003 $320
MiSeq Reagent Kit v3 (150 cycles) MS-102-3001 $300
MiSeq Reagent Kit v3 (600 cycles) MS-102-3003 $400

PhiX control cost $12.75 per uL.

Contact information

For MBCF policy please contact:

Dr. Andrew Doxey

Molecular Core – Director
University of Waterloo
Biology 1, Room 166A
Waterloo, Ontario N2L 3G1
Telephone: (519) 888-4567 ext. 33889
Fax: (519) 746-0614
Email: acdoxey@uwaterloo.ca

For technical and service please contact:

Terence Tang, PhD.
Molecular Core – FACS Specialist
University of Waterloo
Biology 2, Room 357A
Waterloo, Ontario N2L 3G1
Telephone: (519) 888-4567 ext. 33526
Fax: (519) 746-0614
Email: terence.tang@uwaterloo.ca