C. Perry Chou

Professor
C. Perry Chou

Contact information

Phone: 519-888-4567 x43310
Location: E6 4004

Website

C. Perry Chou

Biography summary

C. Perry Chou received his BSc and MSc from National Taiwan University (Taipei, Taiwan) and PhD from Rice University (Houston, USA), all in Chemical Engineering. He is a full professor in the Chemical Engineering Department with a cross appointment in Biology at University of Waterloo. He is primarily involved in developing integrated biochemical, genetic, and metabolic engineering strategies to enhance biomanufacturing with various microorganisms as cell factories. His multidisciplinary expertise in fundamental biological sciences and applied biochemical engineering critically mediates the development of innovative strategies in biomanufacturing and seamlessly integrates various techniques for bioprocess development, including upstream genetic manipulation for microbial strain construction, midstream fermentation technology for microbial cell cultivation, and downstream processing for bioproduct harvest and purification. He is an author of 90+ papers in various peer-review bioscience and bioengineering journals. He has received several awards, in particular Canada Research Chair (Canada) and 1000-talent Award (China). He has been involved in various editorial services, including an editor for Biotechnology Advances, a guest editor for Bioresource Technology, as well as an editorial board member for several peer-review journals such as Scientific Reports.

Research interests

  • biochemical engineering
  • bioprocessing
  • biomanufacturing
  • recombinant protein production
  • microbial biotechnology
  • gene expression
  • genetic engineering
  • metabolic engineering
  • protein engineering
  • biofuel
  • molecular biology

Education

  • 1995, Doctorate, Chemical Engineering, Rice University (Houston, USA)
  • 1987, Master's, Chemical Engineering, National Taiwan University (Taipei, Taiwan)
  • 1984, Bachelor's, Chemical Engineering, National Taiwan University (Taipei, Taiwan)

Selected/recent publications

  • D. Miscevic, J.-Y. Mao, D. Abedi, M. Moo-Young, and C. P. Chou, "Strain engineering for high-level 5-aminolevulinic acid production in Escherichia coli", Biotechnology and Bioengineering, 118:30-42 (2021)
  • D. Miscevic, K. Srirangan, S. Kilpatrick, D. A. Chung, M. Moo-Young, and C. P. Chou, "Heterologous production of 3-hydroxyvalerate in engineered Escherichia coli", Metabolic Engineering, 61, 141-151 (2020)
  • D. Miscevic, J.-Y. Mao, M. Moo-Young, and C. P. Chou, "High-level heterologous production of propionate in engineered Escherichia coli", Biotechnology and Bioengineering, 117, 1304-1315 (2020)
  • V. Akbari, C. P. Chou, and D. Abedi, "New insights into affinity proteins for HER2-targeted therapy: beyond trastuzumab", BBA - Reviews on Cancer, 1874, 188448 (2020)
  • A. Westbrook, D. Miscevic, S. Kilpatrick, M. Bruder, M. Moo-Young, and C. P. Chou, "Strain engineering for microbial production of value-added chemicals and fuels from glycerol", Biotechnology Advances, 37, 538-568 (2019)
  • A. Anand, B. Unnikrishnan, S.-C. Wei, L. Zhang, C. P. Chou, and C.-C. Huang "Graphene oxide and carbon dots as broad-spectrum antimicrobial agents - A minireview", Nanoscale Horizons, 4, 117-137 (2019)
  • A. Westbrook, X. Ren, Jaewon Oh, M. Moo-Young, and C. P. Chou, "Metabolic engineering to enhance heterologous production of hyaluronic acid in Bacillus subtilis", Metabolic Engineering, 47, 401-413 (2018)
  • A. Westbrook, X. Ren, M. Moo-Young, and C. P. Chou, "Engineering of cell membrane to enhance heterologous production of hyaluronic acid in Bacillus subtilis", Biotechnology and Bioengineering, 115, 216-231 (2018)
  • K. Srirangan, M. Bruder, L. Akawi, D. Miscevic, S. Kilpatrick, M. Moo-Young, and C. P. Chou, "Recent advances in engineering propionyl-CoA metabolism for microbial production of value-added chemicals and biofuels", Critical Reviews in Biotechnology, 37:6, 701-722 (2017)
  • K. Srirangan, X. Liu, L. Akawi, M. Bruder, M. Moo-Young, and C. P. Chou, "Engineering Escherichia coli for microbial production of butanone", Applied and Environmental Microbiology, 82, 2574-2584 (2016)
  • K. Srirangan, X. Liu, T. T. Tran, M. Moo-Young, and C. P. Chou, "Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources", Scientific Reports, 6, 36470 (2016)
  • M. E. Pyne, M. Bruder, M. Moo-Young, D. A. Chung, and C. P. Chou, "Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium", Scientific Reports, 6, 25666 (2016)
  • M. Bruder, M. E. Pyne, M. Moo-Young, D. A. Chung, and C. P. Chou, "Extending CRISPR-Cas9 technology from genome editing to transcriptional engineering in Clostridium", Applied and Environmental Microbiology, 82, 6109-6119 (2016)
  • A. Westbrook, M. Moo-Young, and C. P. Chou, "Development of a CRISPR-Cas9 toolkit for comprehensive engineering of Bacillus subtilis", Applied and Environmental Microbiology, 82:4876-4895 (2016) (This paper was included in the Spotlight of the issue.)
  • M. E. Pyne, X. Liu, M. Moo-Young, D. A. Chung, and C. P. Chou, "Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum", Scientific Reports, 6, 26228 (2016)
  • M. E. Pyne, S. Sokolenko, X. Liu, K. Srirangan, M. R. Bruder, M. G. Aucoin, M. Moo-Young, D. A. Chung, and C. P. Chou, "Disruption of the reductive 1,3-propanediol pathway triggers production of 1,2-propanediol for sustained glycerol fermentation by Clostridium pasteurianum", Applied and Environmental Microbiology, 82, 5375-5388 (2016) (This paper was included in the Spotlight of the issue.)
  • M. E. Pyne, M. Moo-Young, D. A. Chung, and C. P. Chou, "Coupling the CRISPR/Cas9 system to lambda Red recombineering enables simplified chromosomal gene replacement in Escherichia coli", Applied and Environmental Microbiology, 81, 5103-5114 (2015)
  • M. E. Pyne, M. Bruder, M. Moo-Young, D. A. Chung, and C. P. Chou, "Technical guide for genetic advancement of underdeveloped and intractable Clostridium", Biotechnology Advances, 32, 623-641 (2014)
  • M. E. Pyne, M. Moo-Young, D. A. Chung, and C. P. Chou, "Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: Chromosomal gene disruption of the endogenous CpaAI restriction enzyme", Biotechnology for Biofuels, 7:163 (2014)
  • M. E. Pyne, M. Moo-Young, D. A. Chung, and C. P. Chou, "Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum", Biotechnology for Biofuels, 6: 50 (2013)
  • K. Srirangan, V. Orr, L. Akawi, A. Westbrook, M. Moo-Young, and C. P. Chou, "Biotechnological advances on penicillin G acylase: pharmaceutical implications, unique expression mechanism, and production strategies", Biotechnology Advances, 31, 1319-1332 (2013)
  • K. Srirangan, L. Akawi, X. Liu, A. Westbrook, E. J.M. Blondeel, M. G. Aucoin M. Moo-Young, and C. P. Chou, "Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli", Biotechnology for Biofuels, 6, 139 (2013)
  • V. Orr, L. Zhong, M. Moo-Young, and C. P. Chou, "Recent Advances in Bioprocessing Application of Membrane Chromatography", Biotechnology Advances, 31, 450-465 (2013)

Graduate studies