C. Perry Chou

C. Perry Chou received his BSc and MSc from National Taiwan University (Taipei, Taiwan) and PhD from Rice University (Houston, USA), all in Chemical Engineering. He is a full professor in the Chemical Engineering Department with a cross appointment in Biology at University of Waterloo. He is primarily involved in developing integrated biochemical, genetic, and metabolic engineering strategies to enhance biomanufacturing with various microorganisms as cell factories. His multidisciplinary expertise in fundamental biological sciences and applied biochemical engineering critically mediates the development of innovative strategies in biomanufacturing and seamlessly integrates various techniques for bioprocess development, including upstream genetic manipulation for microbial strain construction, midstream fermentation technology for microbial cell cultivation, and downstream processing for bioproduct harvest and purification. He is an author of 90+ papers in various peer-review bioscience and bioengineering journals. He has received several awards, in particular Canada Research Chair (Canada) and 1000-talent Award (China). He has been involved in various editorial services, including an editor for Biotechnology Advances, a guest editor for Bioresource Technology, as well as an editorial board member for several peer-review journals such as Scientific Reports.

• biochemical engineering
• bioprocessing
• biomanufacturing
• recombinant protein production
• microbial biotechnology
• gene expression
• genetic engineering
• metabolic engineering
• protein engineering
• biofuel
• molecular biology

• 1995, Doctorate, Chemical Engineering, Rice University (Houston, USA)
• 1987, Master's, Chemical Engineering, National Taiwan University (Taipei, Taiwan)
• 1984, Bachelor's, Chemical Engineering, National Taiwan University (Taipei, Taiwan)

• CHE 161 - Engineering Biology
  • Taught in 2016
• CHE 102 - Chemistry for Engineers
  • Taught in 2016
• CHE 562 - Advanced Bioprocess Eng
  • Taught in 2017, 2018
• CHE 765 - Res Tpcs in Biochemical Eng.
  • Taught in 2016, 2017, 2018
• CHE 660 - Biochemical Engineering
  • Taught in 2018, 2020
• CHE 361 - Bioprocess Engineering
  • Taught in 2016, 2017, 2018, 2020

• V. Akbari, C. P. Chou, and D. Abedi, "New insights into affinity proteins for HER2-targeted therapy: beyond trastuzumab", BBA - Reviews on Cancer, 1874, 188448 (2020) (Accepted in 2020)
• D. Miscevic, K. Srirangan, T. Kefale, S. Kilpatrick, D. A. Chung, M. Moo-Young, and C. P. Chou, "Heterologous production of 3-hydroxyvalerate in engineered Escherichia coli", Metabolic Engineering, 61, 141-151 (2020) (Accepted in 2020)
• D. Miscevic, J.-Y. Mao, M. Moo-Young, and C. P. Chou, "High-level heterologous production of propionate in engineered Escherichia coli", Biotechnology and Bioengineering, 117, 1304-1315 (2020) (Accepted in 2020)
• A. Anand, B. Unnikrishnan, S.-C. Wei, L. Zhang, C. P. Chou, and C.-C. Huang "Graphene oxide and carbon dots as broad-spectrum antimicrobial agents - A minireview", Nanoscale Horizons, 4, 117-137 (2019) (Accepted in 2019)
• A. Westbrook, D. Miscevic, S. Kilpatrick, M. Bruder, M. Moo-Young, and C. P. Chou, "Strain engineering for microbial production of value-added chemicals and fuels from glycerol", Biotechnology Advances, 37, 538-568 (2019) (Accepted in 2019)
• A. Westbrook, X. Ren, Jaewon Oh, M. Moo-Young, and C. P. Chou, "Metabolic engineering to enhance heterologous production of hyaluronic acid in Bacillus subtilis", Metabolic Engineering, 47, 401-413 (2018) (Accepted in 2018)
• A. Westbrook, X. Ren, M. Moo-Young, and C. P. Chou, "Engineering of cell membrane to enhance heterologous production of hyaluronic acid in Bacillus subtilis", Biotechnology and Bioengineering, 115, 216-231 (2018) (Accepted in 2018)
• K. Srirangan, M. Bruder, L. Akawi, D. Miscevic, S. Kilpatrick, M. Moo-Young, and C. P. Chou, "Recent advances in engineering propionyl-CoA metabolism for microbial production of value-added chemicals and biofuels", Critical Reviews in Biotechnology, 37:6, 701-722 (2017) (Accepted in 2017)
• M. E. Pyne, X. Liu, M. Moo-Young, D. A. Chung, and C. P. Chou, "Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum", Scientific Reports, 6, 26228 (2016) (Accepted in 2016)
• M. E. Pyne, S. Sokolenko, X. Liu, K. Srirangan, M. R. Bruder, M. G. Aucoin, M. Moo-Young, D. A. Chung, and C. P. Chou, "Disruption of the reductive 1,3-propanediol pathway triggers production of 1,2-propanediol for sustained glycerol fermentation by Clostridium pasteurianum", Applied and Environmental Microbiology, 82, 5375-5388 (2016) (This paper was included in the Spotlight of the issue.) (Accepted in 2016)
• K. Srirangan, X. Liu, L. Akawi, M. Bruder, M. Moo-Young, and C. P. Chou, "Engineering Escherichia coli for microbial production of butanone", Applied and Environmental Microbiology, 82, 2574-2584 (2016) (Accepted in 2016)
• K. Srirangan, X. Liu, T. T. Tran, T. C. Charles, M. Moo-Young, and C. P. Chou, "Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources", Scientific Reports, 6, 36470 (2016) (Accepted in 2016)
• M. E. Pyne, M. Bruder, M. Moo-Young, D. A. Chung, and C. P. Chou, "Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium", Scientific Reports, 6, 25666 (2016) (Accepted in 2016)
• M. Bruder, M. E. Pyne, M. Moo-Young, D. A. Chung, and C. P. Chou, "Extending CRISPR-Cas9 technology from genome editing to transcriptional engineering in Clostridium", Applied and Environmental Microbiology, 82, 6109-6119 (2016) (Accepted in 2016)
• A. Westbrook, M. Moo-Young, and C. P. Chou, "Development of a CRISPR-Cas9 toolkit for comprehensive engineering of Bacillus subtilis", Applied and Environmental Microbiology, 82:4876-4895 (2016) (This paper was included in the Spotlight of the issue.) (Accepted in 2016)
• M. E. Pyne, M. Moo-Young, D. A. Chung, and C. P. Chou, "Coupling the CRISPR/Cas9 system to lambda Red recombineering enables simplified chromosomal gene replacement in Escherichia coli", Applied and Environmental Microbiology, 81, 5103-5114 (2015) (Accepted in 2015)
• M. E. Pyne, M. Moo-Young, D. A. Chung, and C. P. Chou, "Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: Chromosomal gene disruption of the endogenous CpaAI restriction enzyme", Biotechnology for Biofuels, 7:163 (2014)
• M. E. Pyne, M. Bruder, M. Moo-Young, D. A. Chung, and C. P. Chou, "Technical guide for genetic advancement of underdeveloped and intractable Clostridium", Biotechnology Advances, 32, 623-641 (2014)
• M. E. Pyne, M. Moo-Young, D. A. Chung, and C. P. Chou, "Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum", Biotechnology for Biofuels, 6: 50 (2013)
• K. Srirangan, V. Orr, L. Akawi, A. Westbrook, M. Moo-Young, and C. P. Chou, "Biotechnological advances on penicillin G acylase: pharmaceutical implications, unique expression mechanism, and production strategies", Biotechnology Advances, 31, 1319-1332 (2013)
• K. Srirangan, L. Akawi, X. Liu, A. Westbrook, E. J.M. Blondeel, M. G. Aucoin M. Moo-Young, and C. P. Chou, "Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli", Biotechnology for Biofuels, 6, 139 (2013)
• V. Orr, L. Zhong, M. Moo-Young, and C. P. Chou, "Recent Advances in Bioprocessing Application of Membrane Chromatography", Biotechnology Advances, 31, 450-465 (2013)

• Currently accepting applications for graduate students