MASc Oral Exam/ Analysis of Heterogeneities in a 20 L Bioreactor by Danny Kang
Biological systems are essential in biopharmaceuticals, where rising demand requires efficient bioreactor operation. Scaling from lab to industry is limited by gradients from poor mixing, reducing yields through cell stress and adaptation. This work quantified dissolved oxygen, pH, and kLa gradients in a 20 L bioreactor. While cell density and metabolite gradients were inconclusive, metabolic responses were modeled with modified Monod kinetics, which adapted well to differing conditions.