WIN Seminar - Dr. Govind V. Kaigala "Hydrodynamic shaping of sub-nanoliter volumes of liquids on surfaces: from surface patterning to pathology"

In contrast to standard microfluidics, which are typically closed, we are developing a scanning, non-contact microfluidic technology that can shape liquids in the "open space" over surfaces. This technology utilizes a microfluidic probe (MFP) having microfabricated structures for localizing a liquid of interest on a surface using hydrodynamic flow confinement. MFP permits patterning surfaces with proteins and other biomolecules in an additive and subtractive manner, forming gradients on surfaces, and interacting with cells on surfaces. With flow confinement operating at volumes smaller than 1 nanoliter, a few cells can be targeted in a human tissue section for the specific staining of disease markers. Flow confinement and efficient use of chemicals can be further optimized using a concept called "hierarchical" hydrodynamic flow confinement.  I will show how this family of liquid scanning probe devices is evolving as a bioanalytical tool in order to alter the physics and chemistry of biological interfaces at the micrometer to centimeter-length scales. I will also propose concepts pertaining to tissue microprocessing, cell microtechnology and spatially resolved molecular profiling may contribute to the multi-modal analysis of critical samples in the context of pathology and cell biology.