Jean Duhamel

Professor, Chemistry

Research interests: characterization and modeling of polymers; colloidal particles and chain folding using fluorescence


Biography

Professor Jean Duhamel is well recognized for his expertise at applying fluorescence techniques to characterize synthetic or biological macromolecules and their supramolecular assemblies at the nanoscale. DNA, peptides, polypeptides, dendrimers, or polymeric surfactants are examples of the types of macromolecules which are being currently studied in this laboratory.

After graduating with a degree in Chemical Engineering from the Ecole Nationale Superieure des Industries Chimiques (National School for the Chemical Industry, Nancy, France), Duhamel obtained his Ph.D. in Chemical Engineering in 1989 at the Institut National Polytechnique de Lorraine (National Polytechnic Institute of Lorraine, Nancy, France) under the supervision of Professor Jean-Claude Andre where he learned about the fundamentals of steady-state and time-resolved fluorescence. From 1990 to 1993, he was a post-doctoral fellow in the laboratory of Profesor M. A. Winnik at the University of Toronto (Canada) where he learned about how to apply his knowledge of fluorescence to study polymers in solution. In 1993, Duhamel moved to the University of Pennsylvania (Philadelphia, USA) as a post-doctoral fellow under the supervision of Professor P. Lu who taught him how to apply fluorescence to characterize oligonucleotides in solution. In 1996, he was hired as an assistant professor at the University of Waterloo where he moved through the ranks until he became full professor in 2007. He currently holds a Tier 2 Canada Research Chair, he is a Professional Engineer and the Director for the Institute of Polymer Research. He has published over 70 articles in referred journals.

Education

  • PhD, Chemical Engineering, Institut National Polytechnique de Lorraine, 1989

Jean Duhamel

Research

We are characterizing the behavior of polymers in solution using a variety of techniques. Among those techniques, fluorescence is certainly the workhorse of our research. Most polymers are synthesized and fluorescently labeled in the laboratory. The research projects aim at modeling the flexibility of polyolefins and polypeptides using fluorescence decay measurements and applying a blob-based approach. The dynamics of polypeptides random coils and rods are being investigated. The ability of polypeptides at carrying a hydrophobic drug mimic is also determined.

Solutions of associative polymers have peculiar viscoelastic properties which have found applications in the oil and paint industries. We are determining the level of association of water- and oil-soluble associative polymers by fluorescence. It is correlated with the viscoelastic properties of the solutions.

Specific applications: Characterization of chain folding; Characterization of the associative strength of water- and oil-soluble associative polymers; Characterization of the associations between associative polymers and colloidal particles.

Publications

Recent publications include:

  • Fowler, Michael; Hisko, Victoria; Henderson, Jason; Casier, Remi; Li, Lu; Thoma, Janine Lydia; Duhamel, Jean, DiPyMe in SDS Micelles: Artifacts and Their Implications in the Interpretation of Micellar Properties, LANGMUIR, 31(44), 11971-11981, 2015
  • Pirouz, Solmaz; Wang, Yulin; Chong, J. Michael; Duhamel, Jean, Chemical Modification of Polyisobutylene Succinimide Dispersants and Characterization of Their Associative Properties, JOURNAL OF PHYSICAL CHEMISTRY B, 119(37), 12202-12211,2015
  • Pirouz, Solmaz; Duhamel, Jean; Jiang, Sheng; Duggal, Akhilesh, Quantifying the Level of Intermacromolecular Interactions in Ethylene-Propylene Copolymers by Using Pyrene Excimer Formation, MACROMOLECULES, 48(13), 4620-4630, 2015
  • Yang, Bingqing; Duhamel, Jean, Extraction of Oil from Oil Sands Using Thermoresponsive Polymeric Surfactants, ACS APPLIED MATERIALS & INTERFACES, 7(10), 5879-5889, 2015
  • Chen, Shaohua; Duhamel, Jean; Peng, Baoliang; Zaman, Masuduz; Tam, Kam C., Interactions between a Series of Pyrene End-Labeled Poly(ethylene oxide)s and Sodium Dodecyl Sulfate in Aqueous Solution Probed by Fluorescence, LANGMUIR, 30(44), 13164-13175, 2014
  • Zaragoza-Galan, Gerardo; Fowler, Michael; Rein, Regis; Solladie, Nathalie; Duhamel, Jean; Rivera, Ernesto, Fluorescence Resonance Energy Transfer in Partially and Fully Labeled Pyrene Dendronized Porphyrins Studied with Model Free Analysis, JOURNAL OF PHYSICAL CHEMISTRY C, 118(16), 8280-8294, 2014
  • Pirouz, Solmaz; Wang, Yulin; Chong, J. Michael; Duhamel, Jean, Characterization of the Chemical Composition of Polyisobutylene-Based Oil-Soluble Dispersants by Fluorescence, JOURNAL OF PHYSICAL CHEMISTRY B, 118(14), 3899-3911, 2014
  • Duhamel, Jean, Global Analysis of Fluorescence Decays to Probe the Internal Dynamics of Fluorescent ly Labeled Macromolecules, LANGMUIR, 30(9), 2307-2324, 2014
  • Chen, Shaohua; Siu, Howard; Duhamel, Jean, Interactions between Hydrophobically Modified Alkali-Swellable Emulsion Polymers and Sodium Dodecyl Sulfate Probed by Fluorescence and Rheology, JOURNAL OF PHYSICAL CHEMISTRY B, 118(1), 351-361, 2014
  • Farhangi, Shiva; Weiss, Henning; Duhamel, Jean, Effect of Side-Chain Length on the Polymer Chain Dynamics of Poly(alkyl methacrylate)s in Solution, MACROMOLECULES, 46(24), 9738-9747, 2013
  • Chen, Shaohua; Yi, Wei; Duhamel, Jean; Heinrich, Kathleen; Bengtson, Gisela; Fritsch, Detlev, Effect of the Porosity of a Polymer of Intrinsic Microporosity (PIM) on Its Intrinsic Fluorescence, JOURNAL OF PHYSICAL CHEMISTRY B, 117(17), 5249-5260, 2013
  • Fowler, Michael; Siddique, Bushra; Duhamel, Jean, Effect of Sequence on the Ionization Behavior of a Series of Amphiphilic Polypeptides, LANGMUIR, 29(14), 4451-4459, 2013
  • Chen, Shaohua; Duhamel, Jean, Probing the Hydrophobic Interactions of a Series of Pyrene End-Labeled Poly(ethylene oxide)s in Aqueous Solution Using Time-Resolved Fluorescence, LANGMUIR, 29(9), 2821-2834, 2013
  • Fowler, Michael A.; Duhamel, Jean; Bahun, Greg J.; Adronov, Alex; Zaragoza-Galan, Gerardo; Rivera, Ernesto, Studying Pyrene-Labeled Macromolecules with the Model-Free Analysis, JOURNAL OF PHYSICAL CHEMISTRY B, 116(50), 14689-14699, 2012
  • Keyes-Baig, Christine; Mathew, Manoj; Duhamel, Jean, Lateral Distribution of Charged Species along a Polyelectrolyte Probed with a Fluorescence Blob Model, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(40), 16791-16797, 2012
  • Zaragoza-Galan, Gerardo; Fowler, Michael A.; Duhamel, Jean; Rein, Regis; Solladie, Nathalie; Rivera, Ernesto, Synthesis and Characterization of Novel Pyrene-Dendronized Porphyrins Exhibiting Efficient Fluorescence Resonance Energy Transfer: Optical and Photophysical Properties, LANGMUIR, 28(30), 11195-11205, 2012
  • Gelover-Santiago, Adriana; Fowler, Michael A.; Yip, Jamie; Duhamel, Jean; Burillo, Guillermina; Rivera, Ernesto, Unexpected Absorbance Enhancement upon Clustering Dyes in a Polymer Matrix, JOURNAL OF PHYSICAL CHEMISTRY B, 116(21), 6203-6214, 2012
  • Duhamel, Jean, New Insights in the Study of Pyrene Excimer Fluorescence to Characterize Macromolecules and their Supramolecular Assemblies in Solution, LANGMUIR, 28(16), 6527-6538, 2012
  • Duhamel, Jean, Internal Dynamics of Dendritic Molecules Probed by Pyrene Excimer Formation, POLYMERS, 4(1), 211-239, 2012
  • Siu, Howard; Duhamel, Jean, Molar Absorbance Coefficient of Pyrene Aggregates in Water Generated by a Poly(ethylene oxide) Capped at a Single End with Pyrene, JOURNAL OF PHYSICAL CHEMISTRY B, 116(4), 1226-1233, 2012
  • Chen, Shaohua; Duhamel, Jean; Bahun, Greg J.; Adronov, Alex, Quantifying the Presence of Unwanted Fluorescent Species in the Study of Pyrene-Labeled Macromolecules, JOURNAL OF PHYSICAL CHEMISTRY B, 115(33), 9921-9929, 2011
  • Yip, Jamie; Duhamel, Jean; Qiu, Xing Ping; Winnik, Francoise M., Long-Range Polymer Chain Dynamics of Pyrene-Labeled Poly(N-isopropylacrylamide)s Studied by Fluorescence, MACROMOLECULES, 44(13), 5363-5372, 2011
  • Siddique, Bushra; Duhamel, Jean, Effect of Polypeptide Sequence on Polypeptide Self-Assembly, LANGMUIR, 27(11), 6639-6650, 2011
  • Chen, Shaohua; Duhamel, Jean; Winnik, Mitchell A., Probing End-to-End Cyclization beyond Willemski and Fixman, JOURNAL OF PHYSICAL CHEMISTRY B, 115(13), 3289-3302, 2011
  • Keyes-Baig, Christine; Duhamel, Jean; Wettig, Shawn, Characterization of the Behavior of a Pyrene Substituted Gemini Surfactant in Water by Fluorescence, LANGMUIR, 27(7), 3361-3371, 2011
  • Yip, Jamie; Duhamel, Jean; Qiu, Xing Ping; Winnik, Francoise M., Fluorescence studies of a series of monodisperse telechelic alpha,omega-dipyrenyl poly(N-isopropylacrylamide)s in ethanol and in water, CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 89(2), 163-172, 2011
  • Baig, Christine Keyes; Duhamel, Jean, Electron Transfer between Physically Bound Electron Donors and Acceptors: A Fluorescence Blob Model Approach, JOURNAL OF PHYSICAL CHEMISTRY B, 114(44), 13950-13960, 2010
  • Yip, Jamie; Duhamel, Jean; Bahun, Greg J.; Adronov, Alex, A Study of the Dynamics of the Branch Ends of a Series of Pyrene-Labeled Dendrimers Based on Pyrene Excimer Formation, JOURNAL OF PHYSICAL CHEMISTRY B, 114(32), 10254-10265, 2010
  • Siu, Howard; Duhamel, Jean; Sasaki, Darryl Y.; Pincus, Jennifer L., Nanodomain Formation in Lipid Membranes Probed by Time-Resolved Fluorescence, LANGMUIR, 26(13), 10985-10994, 2010
  • Ingratta, Mark; Mathew, Manoj; Duhamel, Jean, How switching the substituent of a pyrene derivative from a methyl to a butyl affects the fluorescence response of polystyrene randomly labeled with pyrene, CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 88(3), 217-227, 2010
  • Teertstra, Steven J.; Lin, Wai Yau; Gauthier, Mario; Ingratta, Mark; Duhamel, Jean, Comparison of the long range polymer chain dynamics of polystyrene and cis-polyisoprene using polymers randomly labeled with pyrene, POLYMER, 50(23), 5456-5466, 2009
  • Ingratta, Mark; Duhamel, Jean, Effect of Time on the Rate of Long Range Polymer Segmental Intramolecular Encounters, JOURNAL OF PHYSICAL CHEMISTRY B, 113(8), 2284-2292, 2009
  • Ingratta, Mark; Duhamel, Jean, Effect of Viscosity on Long-Range Polymer Chain Dynamics in Solution Studied with a Fluorescence Blob Model, MACROMOLECULES, 42(4), 1244-1251, 2009
  • Siu, Howard; Duhamel, Jean, Molar Absorption Coefficient of Pyrene Aggregates in Water, JOURNAL OF PHYSICAL CHEMISTRY B, 112(48), 15301-15312, 2008
  • Shen, Yu; Duhamel, Jean, Micellization and adsorption of a series of succinimide dispersants, LANGMUIR, 24(190), 10665-10673, 2008
  • Zhang, Mingzhen; Duhamel, Jean, Study of maleated ethylene-propylene copolymers by fluorescence: Evidence for succinimide induced polar associations in an apolar solvent, EUROPEAN POLYMER JOURNAL, 44(9), 3005-3014, 2008
  • Ingratta, Mark; Duhamel, Jean, Effect of side-chain length on the side-chain dynamics of alpha-helical poly(L-glutamic acid) as probed by a fluorescence blob model, JOURNAL OF PHYSICAL CHEMISTRY B, 112(30), 9209-9218, 2008
  • Ingratta, Mark; Hollinger, Jon; Duhamel, Jean, A case for using randomly labeled polymers to study long-range polymer chain dynamics by fluorescence, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(29), 9420-9428, 2008
  • Wang, M.; Adikane, H. V.; Duhamel, J.; Chen, P., Protection of oligo deoxynucleotides against nuclease degradation through association with self-assembling peptides, BIOMATERIALS, 29(8), 1099-1108, 2008

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